مجله دانشگاه علوم پزشکی اراک
سال بیست و سوم شماره 3 (پیاپی 145، امرداد و شهریور 1399)

Tendon, like the skeletal muscle, exhibits mechanical and morphological adaptations resulted from exercise training; however, little is known about the basic cellular and molecular mechanisms that regulate these responses. The aim of the present study was therefore to investigate the effect of 6 weeks of aerobic training on the TGF-β1, myostatin and MMP9 mRNAs expression in the tendon of fast- and slow-twitch muscles. 

Methods & Materials :

For this purpose, 12 male Wistar rats at 8 weeks of age were randomly divided into two groups: experimental (n=6) and control (n=6). The exercise group performed aerobic training for 6 weeks, 5 sessions per week. Forty-eight hours after the last training session, all rats were sacrificed and the tendons of soleus and Extensor Digitorum Longus (EDL) muscles were extracted. Expression of TGF-β1, myostatin and MMP9 mRNAs were assayed using RealTime-PCR. Independent t-test was also used for statistical analysis.

Ethical Considerations: 

All stages of the study were conducted according to the ethical guidelines and authorization of Research Deputy of Islamic Azad University, Central Tehran Branch No. IR.IAU.PS.REC.1398.296. 

 The results showed that the expression of TGF-β1 mRNA in EDL and soleus tendons significantly increased (P≤0.001), whereas the expression of myostatin in EDL tendon was significantly reduced (P≤ 0.001). Increased mRNA expression of MMP9 in the tendon of EDL and soleus muscles was not statistically significant (P>0.05).

 It seems that aerobic exercise can modulate the expression of genes involved in the regulation of tendon collagen in a muscle type-dependent manner.

Staphylococcus aureus is common pathogens of nosocomial infections. Nasal swabs in hospital staff is main sources of hospital infections are considered. The aim of this study was to determine the frequency of nasopharyngeal carriers of methicillin-resistant Staphylococcus aureus and microbial contamination of health care workerschr('39') cell phones in Emergency department of Vali-e-Asr Hospital in Arak City.

Methods & Materials:

 In this descriptive study, nose swabs and cell phone levels were taken from 70 health care workers in the emergency ward of Vali-e-Asr Hospital. The Staphylococcus aureus clinical isolates were identified using standard microbiological methods (catalase, coagulase, mannitol fermentation and DNase). The susceptibility to oxacillin and cefoxitin was detected by the disk diffusion and the mecA genes in this clinically isolated strain of staphylococci was investigated by Polymerase Chain Reaction (PCR).

Ethical Considerations: 

This study was approved by the Research Ethics committee of Arak University of Medical Sciences. (Code : IR.ARAKMU.REC.1396.282).

 According the results, Staphylococcus aureus was isolated in 16 cases, which 5 cases were methicillin-sensitive S. aureus (MSSA), and 11 cases were Methicillin resistant S. aureus (MRSA). Also, 3 cell phones were infected with Staphylococcus aureus, which 1 case was MRSA and 2 cases were MSSA.

 The results of this study showed that frequency of MSSA strains is significant in emergency personnel of the hospital. Thus, regarding to the risk of epidemics due to nosocomial infections, periodic testing for the identification and treatment of carriers among employees for controlling and preventing hospital infections seems necessary.

Background and Aim:

 Apoptosis or programmed death is active biological process and reversible that the balance between growth and cell death in various tissues, particularly myocardial tissue is essential role. The aim of this study was the effect of 8 weeks High Intensity Interval Training (HIIT) with and without food restriction on gene expression of Caspase 3 and 9 in rats.

Methods & Materials: 

This study was experimental multi group design with control group which conducted on 30 rats with two months old male. Subjects were divided in to five homogenous groups including base control, control, caloric restriction, interval exercise training and caloric restriction+interval exercise training. Training groups five sessions in weeks were participated in interval exercise training for 8 weeks. The level of gene expression of the proteins Caspase 3 and 9 was evaluated by Real Time-PCR. Dates were analyzed using the 1-way ANOVA at the level of (P<0.05).

Ethical Considerations: 

The protocol of this study was approved by the Medical Committee of Sanandaj University of Medical Sciences (Code: IR.MYK.REC.1397.5022).

 The results showed that the training group had a significant decrease in gene expression of Caspase 3 in comparison with caloric restriction+exercise training (P<0.05). Also, exercise training and exercise training+caloric restriction significantly decrease in the gene expression of Caspase 9 compared to caloric restriction, base control and control (P<0.05) and significantly decrease in the gene expression of Caspase 3 compared to base control and control (P<0.05).

 According to the results, it seems that high intensity interval training with and without calorie restriction provides the necessary adaptations to inhibit or stop apoptosis induced by aerobic exercise.

Coronary Heart Disease (CAD) is one of the most important causes of mortality. Exercise activities after coronary artery bypass surgery increase the density of myocardial capillaries called angiogenesis and improve cardiovascular function. The aim of the present study was to determine the effect of aerobic-resistance training on plasma basic fibroblast grown factor levels in post-coronary artery bypass grafting patients.

Methods & Materials: 

The sample consisted of 30 men aged 45-60 years who underwent bypass surgery. They were randomly divided into experimental (n=15) and control (n=15) groups. The experimental group performed aerobic resistance training for eight weeks (3 sessions per week) and the control group did not. Blood samples were taken 24 hours before the start of the first training session and 48 hours after the last training session while all subjects were fasting. In vitro ELISA method was used to measure BFGF. Independent and paired t-test and Shapiro-Wilk test were used to analyze the data at the significant level of 0.05 in SPSS v. 21.

Ethical Considerations:

 This study was registered (Ethics Code: IR.IAU.NEYSHABUR.REC.1398.01) in the Ethics Committee of Islamic Azad University, Neishabour Branch, and with Clinical Trial Code: IRCTID:IRCT20191228045919N1.

 Eight weeks of aerobic-resistance training significantly increased bFGF levels in the exercise group compared to the control group (P=0.002). Also, pre and post changes in the experimental group showed a significant increase in plasma bFGF levels (P=0.002), which was not significant in the control group (P=0.758).

 According to the results, it may be concluded that combined (aerobic resistance) exercise may increase angiogenesis and capillary density in post-CABG patients by increasing plasma bFGF levels

Epilepsy is a common brain disorder. Brain function can be affected by the intestinal microflora. The intestinal microflora have a major role in modulating immune responses, producing essential metabolites and mediating neurotransmitters. Antibiotics can affect and reduce these roles and functions by eliminating the intestinal microflora. The aim of this study was to investigate the effect of intestinal natural micro flora removal on seizure susceptibility and seizure behavior modification with the use of probiotics in male Wistar rats.

Methods & Materials:

 This study was performed on 32 male Wistar rats with weight range 200-250 gr. The animals were randomly divided into four groups: 1. Control group; 2. Antibiotic group; 3. Probiotic group and 4. Antibiotic + probiotic group. To remove the microflora, antibiotics (neomycin, ampicillin, and metronidazole) for three weeks and for replacement of microflora, probiotics (Lactobacilli casei, Lactobacillus acidophilus and Bifidobacterium bifidum) for four weeks were administered. Seizures were performed by intraperitoneal injection of pentylentrazole. The microflora was examined by the MRS Agar medium and the Pure Plate method. The data were statistically analyzed in Graph Pad Prism V. 8.

Ethical Considerations:

 This study was approved by the Research Ethics Committee of Arak University of Medical Sciences (Code: IR.ARAKMU.REC.1395.176).

 The use of antibiotics lead to decrease the number of intestinal bacteria (P<0.0001), increased the severity and stability of seizure stages (P<0.05) and decreased the time delay of seizure onset (P<0.05) compared to the control group. Probiotic consumption by modifying the intestinal microflora (P<0.0001) reduced the severity of seizure and increased the time delay of seizure onset (P<0.05).

 Elimination of microflora has the potential to induce seizures, which can be compensated by administration of probiotics.

The deaths caused by traffic accidents are one of the three leading causes of death in Iran. Considering that all deaths from traffic accidents in the forensic organization are undergoing autopsy and also in the treatment centers under oral autopsies, the cause of death is determined, we determined that the cause of reported death from oral autopsy and medical autopsy Compare the law.

Methods & Materials: 

In this descriptive study, patients who were admitted to the forensic medical center during the year 1395 admitted to the Vali-e-Asr Hospital in Arak, were referred. The causes of death in Vali-asr Hospital were evaluated and compared with the outcome of the forensic oral hearing. 

Ethical Considerations:

 This study ethcally approved by the ethics committee of the Arak University of Medical Sciences (Code: IR.ARAKMU.REC.1395.226).

In this study, 109 patients (1.33%) died from a total of 8153 patients with traffic accidents referred to Valiasr hospital in Arak. Of these, 68.8% were male and 31.2% were women. The Mean±SD age of the patients died was 44.03±22.43 years. Also, 59.6% of the dead died during the first 24 hours of the accident. The most common cause of death, according to the results of oral autopsy, was “multiple injuries,” accounting for 49.5% of the patients in 45 patients.

 Abundance of deaths among patients with road traffic accidents and they referred to Valiasr hospital, which was 1.33%. According to the existing standards and considering that the mortality rate in this center is less than 1.5%. It seems that the level of service delivery in this center is favorable.

Lip reading is extraction of speech data from the activity of the lower part of face, particularly jaws, lips, tongue, and teeth that is a natural skill in people with hearing loss. The main purpose of lip reading is to increase the independence of people with hearing loss. Looking at the speakers facial movements significantly increase the ability to understand the spoken words, particularly in the environments where noise is present. In fact this reflects the important role of visual signals. In current study we consider the effects of education on lip reading ability by planning and instructing a lip reading program in male subjects.

Methods & Materials: 

Sara lip reading test 1 and 2 were used for the assessment of lip reading skills and for studying the effects of lip reading training on recognition of speech. This intervention as a semi-experimental study, was conducted on 27 male students, selected by available sampling. For data analysis, statistical analysis of variance with repeated measurements and Mcnemar was used.

Ethical Considerations: 

This research with the code IR.SBMU.REC.1394.144 has been approved by the ethics committee of Shahid Beheshti University of Medical Sciences.

 There were significant differences between Sara lip reading test 1 scores in 2 positions (P=0.000).Lip reading test scores also showed significant differences in Sara lip reading test 2 in 2 positions (P=0.000). Also between the scores of Consonant-vowel, consonant-vowel syllables, in the position before teaching lip-reading and then ,was significant difference (P<0.05).

 The findings of this study showed that instructing lip reading has a positive effect on lip-reading ability in people.

Fertility pattern is one of the most important determinants of population fluctuations. Various individual and social factors affect this pattern. This study aimed to determine the factors affecting the fertility pattern using the structural equation model.

Methods & Materials:

 In this cross-sectional study, 384 women were randomly selected from married women of childbearing age who referred to Taleghani, Valiasr, and Amir Al-Momenin hospitals in Arak as patients. After obtaining verbal consent to participate in the study, a researcher-made and valid questionnaire was completed for them. Structural equation modeling was used to measure the effect of important individual and social variables on fertility patterns. Data analysis and analysis were performed with AMOUS software version 11 and SPSS V. 22.

Ethical Considerations: 

This study was registered with the ethics code IR.ARAKMU.REC.1396.4 by the Research Ethics Committee of Arak University of Medical Sciences.

 The Mean±SD age of the participants in the study was 40.03±11.19. The variables affecting the fertility pattern using the structural equation model included, respectively, the type of individual exercise, the method of contraception, the type of infertility treatment, and the duration of use of the contraceptive method.

 The results showed that physical activity, contraceptive method, and duration of use of this method and the type of infertility treatment were related to fertility pattern. By recognizing the factors affecting the fertility pattern and increasing the awareness of young couples, it is possible to help improve the fertility pattern.

Cervical Cancer (CC) is the third most common malignancy in the women, the main cause of which is human papillomavirus (HPV). Both E6 and E7 oncogenes of the virus play an important role in its tumorigenesis. Today, methods available for screening CC are not capable of detecting the disease at an early stage. Therefore, it is important to identify new biomarkers for early detection of this cancer. For this purpose, in the present study, miRNAs targeting the two oncogenes E6 and E7 of human papillomavirus (types 16 and 18) were studied in CC by bioinformatics.

Methods & Materials:

 First, using the NCBI database, the E6 and E7 gene sequences were obtained for both human papillomavirus types 16 and 18. Then, using the miRBase and RNA22 bioinformatics databases, the most appropriate targeting miRNAs for these genes were selected.

Ethical Considerations:

 This study was approved by Ethics Committee of Arak University of Medical Sciences.

 Based on the P obtained from bioinformatics databases, miRNA including miR-92a-5p (P=7.51e-2), miR-195-3p (P=2.24e-1), miR-34a-5p (P=2.73e-1) and miR-155-5p (P=4.95e-2) were introduced for the two genes E6 and E7.

 Results from bioinformatics studies revealed that of the four miRNAs identified, miR-155-5p and miR-92a-5p are probably the targeting miRNAs specific for the E6 and E7 genes, respectively. Therefore, it seems that these miRNAs can be a suitable candidate for in vitro studies in CC patients.

Exercise aerobic is associated with increased creatine kinase and blood lactate immediately after exercise, leading to increased muscle damage and undesirable changes in many cellular markers including serum creatine kinase. In such situations, consuming nutrients and supplements such as coenzyme Q10 may prevent metabolic stress damage by increasing buffering power. However, comprehensive studies have not been performed on the effects of this nutrient on the index of muscle injury and fatigue caused by exercise.

Methods & Materials:

 For this purpose, 20 inactive volunteer men were randomly divided into two groups of 10-person Coenzyme Q10 supplement (2.5 mg/kg body weight) and quasi-drug (2.5 mg/kg body weight Dextrose). All subjects participated in the Bruce test exercise contract after 14 days of supplementation. Blood sampling was performed in four stages including baseline, after supplementation, immediately after exercise and two hours after exercise. Creatine kinase, lactate and cortisol indices of both groups were measured during these four stages. Data were analyzed by means of standard deviation and repeated measures ANOVA, Bonferroni post hoc and Independent T-test using SPSS V. 17 at the significant level of 0.05.

Ethical Considerations: 

This article has been approved by the ethics committee of Tabriz School of Medical Sciences with the ethics code IRCT 201203104663N8.

 The results showed that 14 days of Coenzyme Q10 supplementation had a significant effect on cortisol level (P<0.05). In addition, one session of exhausting aerobic activity increased creatinine kinase and lactate (P<0.05). On the other hand, creatinine kinase did not differ significantly after exercise (P>0.05). 

 According to the results of the present study, 14-day supplementation of Coenzyme Q10 may reduce the cellular damage induced by exhaustive aerobic activity in inactive men and prevent an increase in blood lactate levels.

Hypertension is a common disease and universal that can cause cardiovascular disease and kidney damage. The purpose of this study was to determine the effect of an eight-weeks massage on blood pressure (systolic and diastolic), heart rate and C-reactive protein in women with hypertension. 

Methods & Materials: 

In this quasi-experimental study, 44 volunteer women with Mean±SD age of 42.12±5.31years were selected from women referred to Arak Oil Company specialized polyclinic with hypertension. Four women excluded from the study for some reason, and the rest were divided into experimental and control groups. In massage group massage was done for three sessions per week, 45-60 minutes in each session, on the back and upper limbs for eight weeks. Data analysis was conducted using dependent and independent t-test by SPSS V. 25 statistical software at the significant level of (P≤0.05).

Ethical Considerations: 

This study (Code: 92-160-26) was approved in Research Ethics Committee of Arak University of Medical Sciences.

 The results showed that eight weeks of massage reduced blood pressure (P=0.001), the heart rate (P=0.001), and C-reactive protein (P=0.001) in women with hypertension compared to the control group (P=0.62).

 The findings of this study showed that massage for eight weeks is an efficient and appropriate method to improve systolic and diastolic blood pressure, heart rate, and C-reactive protein in patients with hypertension.

Myasthenia Gravis (MG) is a neuromuscular disorder and auto-immune disease that is associated with symptoms of fluctuating muscle fatigue and dysfunction of the neuromuscular junction system. This disease greatly affects various aspects of the patientchr('39')s life and impacts the personchr('39')s perception of the quality of life. The aim of this study was to explain and analyze life experiences with myasthenia gravis disease in a young girl.

Methods & Materials: 

This report is a qualitative case with interpretive phenomenological approach that examines the life experiences of a young girl with myasthenia gravis in 2019. Data were collected through semi-structured interviews and analyzed using Van Manenchr('39')s phenomenological approach.

Ethical Considerations:

 This study was registered in the Research Ethics Committee of Khatam University (Code: 3299/100/P/98).

 Thematic analysis indicated that myasthenia gravis disease affects all aspects of personchr('39')s life world. These four aspects include living and feelings with time, body, space, and others. Eventually, three interrelated themes emerged from the patientchr('39')s perception of the world around her, including physical weakness, future ambiguity, and frustration.

 The present study shows a clear image and understanding of the problems of living with MG in a young girl. The phenomenological world of mixed by physical weakness, ambiguity in the future, and frustration that indicating the suffering and distress that the patient experiences following the illness. The results of this study contribute to the awareness of health and rehabilitation specialists in the field of work and research in myasthenia gravis as a neurological disease.